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Learn more about spectral flow cytometry and find the right BD solutions for your workflow.
There is an exponential increase in the number of fluorochromes available for flow cytometry, extending the limits of possibilities for new discoveries. The major limitation to this is the necessity to meticulously match fluorophores to the range of spectrum defined by the filters in order to avoid fluorescence spillover and spread. Spectral flow cytometry overcomes this limitation by allowing exploration of the complete emission spectrum of fluorochromes.

Discover how spectral flow cytometry differs from conventional flow cytometry and how to design your panels for spectral flow cytometry directly from this page.

Introduction to Spectral Flow: Cytometry Fundamentals

Watch this 10-minute video on how to get started and learn:

  • The fundamentals of spectral vs conventional cytometry
  • Autofluorescence unmixing
  • Fluorochrome choice flexibility
  • Increased panel size

Fluorochrome Choice Flexibility

Find out how fluorochromes are chosen and watch this short video for the following key takeaways:

  • Fluorochrome choice only depends on the availability of a suitable laser for fluorochrome excitation
  • Any commercially available fluorochrome excited by the available lasers can be individually detected with no need for filter optimization or swaps
  • Larger fluorochrome selection results in more panel design flexibility
  • Lack of filter requirements also increases flexibility of fluorescent protein detection

Unravel the complexity of the adaptive immune system with the new BD Rhapsody™ Single-Cell TCR/BCR Multiomics Assay. This assay enables profiling of the complete V(D)J region of the T and B cell molecules at the single cell level alongside a targeted or whole transcriptome mRNA workflow with optional protein profiling using BD® AbSeq Antbodies.

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VDJ Brochure

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Discover the Spectrally Enabled BD FACSymphony™ A5 SE Cell Analyzer and S6 SE Cell Sorter

A5 SE
 BD FACSymphony™ A5 SE Cell Analyzer Brochure




BD FACSymphony™ A5 SE Cell Analyzer Technical Specifications

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BD FACSymphony™ S6 Cell Sorter Brochure

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Hospitals and healthcare facilities

BD is a market leader in hospital products that can reduce the incidence of sharps injuries and exposure to bloodborne pathogens. Patient safety has been a focus of BD innovation for years, not only in the United States but also around the world. Working closely with organizations like the International Safety Center (EPINet) is an important part of our efforts to keep patients and workers safe.

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BD® PRIMER Program

Our BD® PRIMER Program (Partnering Reliably in Multicolor Experimental Research) is specially designed to offer you expert-level support, provided by our highly skilled Applications Scientists, to help you develop a well-designed panel and accelerate your time to insight.

Program services include:

  • One-on one experiment design consultations and support
  • Virtual and in-person panel design seminars led by world-renowned thought leaders
  • Predesigned multicolor panels
  • Special panel pricing
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Store Tab

360° rotation knob is effectively sized, contoured and located to enable easy instrument rotation.

360° rotation knob is effectively sized

360° rotation knob is effectively sized

360° rotation knob is effectively sized

More product details

Store Tab

360° rotation knob is effectively sized, contoured and located to enable easy instrument rotation.

360° rotation knob is effectively sized

360° rotation knob is effectively sized

360° rotation knob is effectively sized

More product details

Hospitals and healthcare facilities

BD is a market leader in hospital products that can reduce the incidence of sharps injuries and exposure to bloodborne pathogens. Patient safety has been a focus of BD innovation for years, not only in the United States but also around the world. Working closely with organizations like the International Safety Center (EPINet) is an important part of our efforts to keep patients and workers safe.

Safety syringes and needles >>
Hazardous drug safety >>
Infection prevention >>

Hospitals and healthcare facilities

BD is a market leader in hospital products that can reduce the incidence of sharps injuries and exposure to bloodborne pathogens. Patient safety has been a focus of BD innovation for years, not only in the United States but also around the world. Working closely with organizations like the International Safety Center (EPINet) is an important part of our efforts to keep patients and workers safe.

Safety syringes and needles >>
Hazardous drug safety >>
Infection prevention >>

To continue learning about spectral flow cytometry:

Spectrally enabled BD FACSymphony™ S6 SE Cell Sorter is available in the US through SORP.

E-learning courses

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User group webcasts

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Let's have a conversation

E-learning courses
User group webcasts

Let's have a conversation

E-learning courses
User group webcasts

Let's have a conversation

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REFERENCES

1. Jones M.Pellentesque habitant morbi tristique senectus et netus et malesuada fames ac turpis egestas vestibulum tortor quam.

2. CDC Laboratory quality assurance and standardization programs December 1,2014

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